NIRVANA
Jump to navigation
Jump to search
Introduction
NIRVANA stands for Nanopore sequencing of Isothermal Rapid Viral Amplification for Near real-time Analysis
Indications
Procedure
- uses small, portable device that can screen 96 samples at the same time
- simultaneously test samples for COVID-19, influenza A, human adenovirus, & non-SARS-CoV2 human coronavirus
- including sequences of 5 regions of SARS-CoV2 that captures the B.1.1.7 variant
- within 15 minutes, the device begins to report positive & negative results
- within 3 hours, the device finalizes results of 96 samples
Mechanism of action
- uses isothermal recombinase polymerase amplification (RPA) coupled with real-time nanopore sequencing
- allows for amplifying longer stretches of DNA, & to probe for multiple genes at the same time
Notes
- portable, pocket-sized machine
More general terms
Additional terms
References
- ↑ Salk Institute Fast, portable test can diagnose COVID-19 and track variants. The field test, called NIRVANA, can simultaneously detect and sequence SARS-CoV-2, influenza and other viruses. Science News. March 31, 2021 https://www.sciencedaily.com/releases/2021/03/210331173739.htm
Bi C, Ramos-Mandujano G, Tian Y et al Simultaneous Detection and Mutation Surveillance of SARS-CoV-2 and co-infections of multiple respiratory viruses by Rapid field-deployable sequencing. Med. 2021 March 31. PMID: https://www.ncbi.nlm.nih.gov/pubmed/33821249 PMCID: PMC8011639 Free PMC article - ↑ Cite error: Invalid
<ref>tag; no text was provided for refs namedref2
Cite error: <ref> tag with name "ref2" defined in <references> group "" has no content.