alpha-1-fetoprotein in body fluid
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Reference interval
Principle
The IMx AFP assay is a Microparticle Enzyme Immunoassay (MEIA) for the quantitative measurement of Alpha-fetoprotein (AFP) in serum or plasma.
The IMx AFP reagents & samples are added to the reaction cell in the following sequence:
- The probe/electrode assembly delivers the sample, the specimen diluent & Anti-AFP Coated microparticles into the incubation well of the reaction cell.
- An aliquot of the reaction mixture is transferred to the glass fiber matrix. The microparticles bind irreversible to the glass fiber matrix.
- The matrix is washed to remove unbound materials.
- The Anti-AFP: Alkaline Phosphatase Conjugate is dispensed onto the matrix & binds to the antibody-antigen complex.
- The matrix is washed to remove unbound materials.
- The substrate, 4-Methylumbelliferyl Phosphate, is added to the matrix & the fluorescent product is measured by the MEIA optical assembly.
Clinical significance
Specimen
- Serum, plasma, or amniotic fluid may be used with the IMx AFP assay.
- If assay is performed within 24 hours after collection, the specimen should be stored in the refrigerator at 2-80C. If the testing will be delayed more than 24 hours, the specimen should be frozen. Mix thoroughly after thawing to ensure consistency in the results. Avoid repeated freezing & thawing.
- Specimens showing particulate matter, erythrocytes, or turbidity should be centrifuged before testing.
- Amniotic fluid samples must be diluted prior to performing the IMx AFP assay.
- To quantitate the concentration of AFP in AMNIOTIC FLUID specimens, perform a Manual Dilution Procedure. For Manual Dilution use the IMx AFP Specimen Diluent; make a 1:10 & 1:100 dilutions by adding 50 uL of the sample to 450 uL of diluent, mix thoroughly, in a second tube add 50 uL of the 1:10 dilution to 450 uL of diluent. Mix thoroughly. Multiply the concentration of the diluted sample by the dilution factor.
SAMPLE VOLUME: 150 uL of specimen is the minimum volume require to perform the assay.
More general terms
More specific terms
- alpha-1-fetoprotein (AFP) in amniotic fluid
- alpha-1-fetoprotein (AFP) in cerebrospinal fluid
- alpha-1-fetoprotein (AFP) in cord blood
- alpha-1-fetoprotein (AFP) in peritoneal fluid
- alpha-1-fetoprotein (AFP) in pleural fluid
- alpha-1-fetoprotein (AFP) in serum/plasma
Additional terms
References
- ↑ Abbott Laboratories ,Diagnostic Division ,Abbott Park, IL 60064 Tumor Markers, IMx AFP, FEBRUARY 1990.
- ↑ IMx System Operation Manual, Abbott Diagnostics, a Division of Abbott Laboratories, Abbott Park, IL. 60064, AUGUST 1988.
- ↑ IMx System Assay Manual, Abbott Diagnostics, a Division of Abbott Laboratories, Abbott Park, IL. 60064, AUGUST 1988.
- ↑ Clinical Guide to Laboratory Tests, 3rd ed. Teitz ed., W.B. Saunders, 1995