melanoma antigen recognized by T-cells 1; MART-1; melan-A protein; antigen SK29-AA; antigen LB39-AA (MLANA, MART1)

Function

• plays a role in expression, stability, trafficking, & processing of melanocyte protein SILV/PMEL17, involved in formation of stage II melanosomes
• interacts with SILV/PMEL17

Structure

• acylated

Compartment

• endoplasmic reticulum membrane
• single-pass type 3 membrane protein
• Golgi, trans-Golgi network membrane
• melanosome
• also found in small vesicles & tubules dispersed over the entire cytoplasm
• a small fraction of the protein is inserted into the membrane in an inverted orientation
• inversion of membrane topology results in relocalization of the protein from a predominant Golgi/post- Golgi area to the endoplasmic reticulum
• melanoma cells expressing MLANA with an inverted membrane topology are more effectively recognized by specific cytolytic T-lymphocytes than those expressing MLANA in its native membrane orientation

Expression

• restricted to:
  • retina
  • melanoma
  • melanoma cell lines

Laboratory

• Melan-A Ag in tissue

Notes

see A103 antibody

More general terms

• melanoma associated antigen (MAGE)

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=2315
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:2315
• OMIM: https://mirror.omim.org/entry/605513
• UniProt: http://www.uniprot.org/uniprot/Q16655.html