prolyl 3-hydroxylase 2 (leprecan-like protein 1, myxoid liposarcoma-associated protein 4, LEPREL1, MLAT4, P3H2)

Function

• has prolyl 3-hydroxylase activity
• catalyzes posttranslational formation of 3-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens, especially collagen type 4 & collagen type 5

procollagen L-proline + 2-oxoglutarate + O2
                    <-->
procollagen trans-3-hydroxy-L-proline + succinate + CO2

Cofactor: Fe+2/Fe+3, ascorbate

Structure

• belongs to the leprecan family
• contains 1 PKHD domain (prolyl/lysyl hydroxylase domain)
• contains 4 TPR repeats

Compartment

endoplasmic reticulum, Golgi

Expression

• expressed in heart, placenta, lung, liver, skeletal muscle & kidney
• expression localized to the epithelia of bile ducts & to the sacroplasm of heart muscle & skeletal muscle
• in the pancreas, localized to a subpopulation of Langerhans islet cells
• in the salivary gland, expressed in acinar cells

More general terms

• hydroxylase; monooxygenase
• glycoprotein
• metalloprotein

Database

• UniProt: http://www.uniprot.org/uniprot/Q8IVL5.html