NO synthase-2A (macrophage NO synthase, inducible NO synthase, hepatocyte NOS, NOS2A)

Function

• produces nitric oxide (NO), messenger molecule with diverse functions throughout the body
• in macrophages, NO mediates tumoricidal & bactericidal actions
• regulated by Ca+2/calmodulin
• binds SLC9A3R1

L-arginine + n NADPH + m O2
          <-->
citrulline + nitric oxide + n NADP+

Cofactor:

• heme group (putative)
• binds 1 FAD (putative)
• binds 1 FMN (putative)
• tetrahydrobiopterin (BH4)

Structure

• homodimer
• belongs to the NOS family
• contains 1 flavodoxin-like domain

Alternative splicing

named isoforms=2

Expression

• expressed in the liver, retina, bone cells & airway epithelial cells of the lung
• not expressed in the platelets
• induced by endotoxins & cytokines
• aspirin inhibits expression & function of NOS2A, effects may be exerted at the level of translational/ post-translational modification & directly on the catalytic activity

More general terms

• NO synthase-2 (inducible, i-NOS)
• phosphoprotein

Additional terms

• nitric oxide (NO, endothelium derived relaxation factor {EDRF})

Database

• UniProt: http://www.uniprot.org/uniprot/P35228.html
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:4843
• OMIM: https://mirror.omim.org/entry/163730