indirect antiglobulin test (IAT, indirect Coomb's test)
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Clinical significance
,Agglutination of erythrocytes by specific antihuman antibodies after an in vitro incubation period (when the direct antiglobulin test is negative) indicating that plasma/serum contains anti- erythrocytic antibodies. Depending upon the application, patient, donor or reagent erythrocytes may be used.
Indirect antiglobilin test is an in vitro test for:
- detection & identification of erythrocyte antibodies in sera (type & screen)
- typing of erythrocyte antigens
- cross-matching
A positive indirect antiglobilin test from a patient specimen does NOT make a diagnosis of hemolysis! A positive test may occur as a result of recent transfusion of red blood cells.