LIM domain kinase 2; LIMK-2 (LIMK2)

Function

• serine/threonine kinase
• phosphorylates myelin basic protein & histones in vitro
• binds ROCK1; ROCK1 links Rho GTPases to LIMK2
• phosphorylated on Ser &/or Thr by ROCK1
• activation of TGFBR1 leads to regulation of actin assembly, via LIMK2
• binds LKAP
• interacts with PARD3
• interacts with NISCH (putative)

ATP + a protein <--> ADP + a phosphoprotein

Structure

• belongs to the protein kinase superfamily, TKL Ser/Thr protein kinase family
• contains 2 LIM Zn+2-binding domains
• contains 1 PDZ domain (DHR domain)
• contains 1 protein kinase domain

Compartment

• isoform LIMK2a: cytoplasm, nucleus
• isoform LIMK2b: mainly in the cytoplasm, infrequently translocated to the nucleus

Alternative splicing

named isoforms=2, LIMK2a, LIMK2b

Expression

• expressed in placenta > liver, lung, kidney, & pancreas
• LIMK2a is more abundant then LIMK2b in liver, colon, stomach, & spleen
• in brain, kidney, & placenta LIMK2b is the dominant form
• in adult lung, both LIMK2a & LIMK2b is nearly equally observed

More general terms

• LIM domain protein
• serine/threonine kinase

Database

• UniProt: http://www.uniprot.org/uniprot/P53671.html
• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=3985
• OMIM: https://mirror.omim.org/entry/601988