chloride intracellular channel protein 1; chloride channel ABP; nuclear chloride ion channel 27; NCC27; regulatory nuclear chloride ion channel protein; hRNCC (CLIC1, G6, NCC27)
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Function
- can insert into membranes & form chloride ion channels
- channel activity depends on pH
- membrane insertion seems to be redox-regulated & may occur only under oxidizing conditions
- involved in regulation of the cell cycle
- hydrogen peroxide treatment causes a conformation change, leading to dimerization & formation of an intramolecular disulfide bond between Cys-24 & Cys-59
- monomer, homodimer (in vitro)
- interacts with TRAPPC2
- dimerization requires a conformation change that leads to the exposure of a large hydrophobic surface
- in vivo, this may lead to membrane insertion
- interacts with AKAP9
Structure
- members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane & functions as chloride channel
- a conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion
- belongs to the chloride channel CLIC family
- contains 1 GST C-terminal domain
Compartment
- nucleus. nuclear membrane
- single-pass membrane protein (probable)
- cytoplasm, cell membrane
- mostly in the nucleus including in the nuclear membrane
- small amount in the cytoplasm & the plasma membrane
- exists both as soluble cytoplasmic protein & as membrane protein with probably a single transmembrane domain
Expression
Notes
- seems to have very low affinity for glutathione, even though glutathione binding was observed in protein crystals