glutamine-dependent NAD+ synthetase; NAD+ synthase [glutamine-hydrolyzing]; NAD+ synthetase 1 (NADSYN1)

Function

• NAD biosynthesis
• homohexamer

ATP + deamido-NAD(+) + L-glutamine + H2O
           <-->
AMP + diphosphate + NAD+ + L-glutamate

Kinetic parameters:

• KM=0.49 mM for deamido-NAD+
• KM=0.089 mM for ATP
• KM=1.44 mM for glutamine
• KM=13.1 mM for ammonium
• Vmax=0.99 nmol/min/ug enzyme deamido-NAD+
• Vmax=0.61 nmol/min/ug enzyme ATP
• Vmax=0.70 nmol/min/ug enzyme glutamine

Structure

• homohexamer
• in C-terminal section; belongs to NAD synthetase family
• contains 1 CN hydrolase domain

More general terms

• ligase or synthetase

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=55191
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:55191
• OMIM: https://mirror.omim.org/entry/608285
• UniProt: http://www.uniprot.org/uniprot/Q6IA69.html