lysine-specific histone demethylase 1; amine oxidase flavin-containing domain protein 2; BRAF35-HDAC complex protein; BHC110 (AOF2, KDM1A, KIAA0601, LSD1)

Function

• histone demethylase 2 demethylates Lys-4 of histone H3
  • demethylates both mono- & tri-methylated Lys-4 of histone H3
• specific tag for epigenetic transcriptional activation (acts as a corepressor)
• demethylates Lys-9 of histone H3
• specific tag for epigenetic transcriptional repression
  • derepression of androgen receptor target genes
• FAD-mediated substrate oxidation to generate imine that is subsequently hydrolyzed
• repression of neuronal genes (putative)
• requires the presence of RCOR1/CoREST for activity
• component of a BHC histone deacetylase complex

Cofactor: FAD

Kinetic parameters:

• KM=3.0 uM for H3 monomethyl-K4
• KM=4.2 uM for H3 dimethyl-K4
• KM=3.9 uM for H3 monomethyl-K4-monomethyl-K9
• KM=17.5 uM for monomethyl-K4-acetyl-K9

Structure

• SWIRN domain may act as an anchor site for a histone tail

Compartment

nucleus

Alternative splicing

named isoforms=2

Expression

ubiquitously expressed

More general terms

• oxidoreductase
• histone demethylase

Component of

• BHC histone deacetylase complex

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=23028
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:23028
• OMIM: https://mirror.omim.org/entry/609132
• UniProt: http://www.uniprot.org/uniprot/O60341.html