A disintegrin & metalloproteinase with thrombospondin type 1 motif 12; ADAMTS-12; ADAM-TS 12; ADAM-TS12; (ADAMTS12)

Function

• interaction with the extracellular matrix
• precursor is cleaved by a furin endopeptidase
• subjected to an intracellular maturation process yielding a 120 kD N-terminal fragment containing the metalloproteinase, disintegrin, one TSP type-1 domain & the Cys-rich domains & a 83 kD C-terminal fragment containing the spacer 2 & four TSP type-1 domains

Cofactor: binds 1 Zn+2 per subunit (putative)

Structure

• spacer domain & TSP type-1 domains are important for a tight interaction with the extracellular matrix
• conserved cysteine present in the cysteine-switch motif binds the catalytic Zn+2, thus inhibiting the enzyme
• dissociation of Cys from Zn+2 upon the activation- peptide release activates the enzyme
• contains 1 disintegrin domain
• contains 1 peptidase M12B domain
• contains 1 PLAC domain
• contains 8 TSP type-1 domains

Compartment

• secreted, extracellular space, extracellular matrix (putative)

Alternative splicing

named isoforms=2

Expression

expressed exclusively in fetal lung

Pathology

• expressed in gastric carcinomas & in cancer cells of diverse origin

More general terms

• ADAMTS protease
• glycoprotein
• phosphoprotein

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=81792
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:81792
• OMIM: https://mirror.omim.org/entry/606184
• UniProt: http://www.uniprot.org/uniprot/P58397.html