protein kinase C-theta (PRKCQ, PRKCT)
Jump to navigation
Jump to search
Function
- Ca+2-independent, phospholipid-dependent,
- serine/threonine-specific enzyme
- activated by diacylglycerol
- essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development
- links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes
- required for interleukin-2 (IL2) production
- interacts with TXNL2/PICOT
- 3 specific sites;
- Thr-538 (activation loop of the kinase domain)
- Ser-676 (turn motif)
- Ser-695 (hydrophobic region) need to be phosphorylated for its full activation
- autophosphorylation at Thr-219 is required for targeting to the TCR & cellular function of PKC upon antigen receptor ligation
Structure
- C1 domain, containing the phorbol ester/DAG-type region 1 is the diacylglycerol sensor
- C2 domain is a non-Ca+2 binding domain
- belongs to the protein kinase superfamily, AGC Ser/Thr protein kinase family, PKC subfamily
- contains 1 AGC-kinase C-terminal domain
- contains 1 C2 domain
- contains 2 phorbol-ester/DAG-type Zn+2 fingers
- contains 1 protein kinase domain
Alternative splicing
named isoforms=2
Expression
skeletal muscle, megakaryoblastic cells, platelets