E3 ubiquitin-protein ligase MID2; midin-2; midline defect 2; midline-2; RING finger protein 60; tripartite motif-containing protein 1 (MID2, FXY2, RNF60, TRIM1)

Function

• protein modification; protein ubiquitination
• phosphorylated on Ser & Thr
• homodimer or heterodimer with MID1
• interacts with IGBP1

Structure

• the tripartite motif (RBCC; RING- & B box-type Zn+2 fingers & coiled coil domains) mediates dimerization
• association with microtubules is dependent on the C-terminal B30.2 domain
• belongs to the TRIM/RBCC family
• contains 2 B box-type Zn+2 fingers
• contains 1 B30.2/SPRY domain
• contains 1 COS domain
• contains 1 fibronectin F3 module
• contains 1 RING-type Zn+2 finger

Compartment

-cytoplasm, cytoskeleton; microtubule-associated

Alternative splicing

named isoforms=2

Expression

• low level in fetal kidney & lung, & in adult prostate, ovary & small intestine

Notes

• uncertain whether Met-1 or Met-21 is the initiator

More general terms

• midline protein
• tripartite motif-containing protein (trim protein)
• E3 ubiquitin ligase

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=11043
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:11043
• OMIM: https://mirror.omim.org/entry/300204
• UniProt: http://www.uniprot.org/uniprot/Q9UJV3.html