endonuclease 8-like 3; DNA glycosylase FPG2; DNA glycosylase/AP lyase Neil3; endonuclease VIII-like 3; Nei-like protein 3 (NEIL3)

Function

• DNA glycosylase activity is uncertain (controversial)
• may prefer ssDNA or partially ssDNA structures such as bubble & fork structures to dsDNA in vitro
• broad recognition spectrum, preferring FapyA & FapyG > 5-OHU, 5-PHC & 5-OHMH > Tg & 8-oxoA
  • no activity on 8-oxoG
• removes damaged bases from DNA, leaving an abasic site
• the C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar & a product with a terminal 5'-phosphate

Structure

• belongs to the FPG family
  • lacks a Pro at the N-terminus which functions as an active site residue in other members of the FPG family
• contains 1 FPG-type Zn+2 finger
• contains 1 RanBP2-type Zn+2 finger

Compartment

nucleus

Expression

• detected in thymus, pancreas & testis
• expressed at higher levels in tumor tissue than in the corresponding normal tissues except for pancreas & testis where expression is higher in normal than tumor tissue

More general terms

• DNA glycosylase
• DNA-binding protein
• zinc finger protein

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=55247
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:55247
• OMIM: https://mirror.omim.org/entry/608934
• UniProt: http://www.uniprot.org/uniprot/Q8TAT5.html