elastin, collagen & total protein in tissue

Indications

• assessment of tissue levels of elastin & collagen

Procedure

• tissue sampling
• acid hydrolysis of tissue breaking proteins down into amino acids
• quantify amino acids unique to each protein
  • desmosine for elastin, hydroxyproline for collagen
• total protein, useful for normalization, assessed by measuring free amino groups
• measurements are performed using 96-well assay plates requiring only a plate reader to determine absorbance

More general terms

• special chemistry test