CSF real time quaking-induced conversion assay
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Indications
- evaluation of prion disease
Clinical significance
- most sensitive & specific test for prion proteins in the CSF
Methods
- uses recombinant prion protein & thioflavin T fluorescence to detect small quantities of prion protein in CSF[1]
- prion protein in the CSF induces the recombinant prion protein to change conformation & form fibrils that bind thioflavin T & begin to fluoresce