HIV1 Ab in serum/plasma/blood; (HIV1 EIA/ELISA)

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EIA is the most common method for screening individuals & blood products for antibodies to HIV. Antibodies usually appear by week 4-12 of infection, but seronegativity may be prolonged.

Additionally, antibodies may diminish as the disease process progresses. Thus a negative EIA does not rule out disease.

EIA's for HIV are 99+% sensitive for antibody to the virus, but false positives may occur with specimens that are RPR positive or from patients with hemophilia or on dialysis. EIA is generally done in duplicate & duplicate negatives are considered negative. Positive EIA's are confirmed by western blot.


More general terms

Additional terms


  1. Clinical Guide to Laboratory Tests, 3rd edition, NW Tietz ed, WB Saunders, Philadelphia, 1995
  2. HIV-1 Antibody Confirm, Western Blot Laboratory Test Directory ARUP:
  3. HIV-1 Antibody Laboratory Test Directory ARUP: