lactate dehydrogenase (LDH) in body fluid
Principle
The Kodak Ektachem Clinical Chemistry Slide (LDH) is a dry, multilayered, self-contained analytical element coated on a clear polyester support. The assay for lactate dehydrogenase (LDH) uses pyruvate & NADH as substrates to generate lactate & NAD.
An 11 microliter drop of sample is deposited on the slide & evenly distributed by the spreading layer. Lactate dehydrogenase catalyzes the conversion of pyruvate & NADH to lactate & NAD. The oxidation of NADH, which is monitored by reflectance spectrophotometry, is used to measure lactate dehydrogenase activity.
Preparation: No special patient preparation is necessary.
Clinical significance
Lactate dehydrogenase is an enzyme present in the cytosol of the human cells; it catalyzes the reversible reduction of pyruvate to lactate using NADH.
Specimen
More general terms
More specific terms
- lactate dehydrogenase (LDH) in amniotic fluid
- lactate dehydrogenase (LDH) in CSF
- lactate dehydrogenase (LDH) in dialysis fluid
- lactate dehydrogenase (LDH) in gastric fluid
- lactate dehydrogenase (LDH) in pericardial fluid
- lactate dehydrogenase (LDH) in peritoneal fluid
- lactate dehydrogenase (LDH) in pleural fluid
- lactate dehydrogenase (LDH) in semen
- lactate dehydrogenase (LDH) in serum
- lactate dehydrogenase (LDH) in synovial fluid
- lactate dehydrogenase (LDH) in urine
Additional terms
References
- ↑ Kodak Ektachem 700 Test Methodologies Manual, Kodak Clinical Products, Rochester, N.Y., 1990.
- ↑ Kodak Ektachem 700 Operators Manual, Kodak Clinical Products, Rochester, N.Y., 1987.
- ↑ Package Insert, Kodak Ektachem Special Calibrators, Kodak Clinical Products, Rochester, N.Y., 1985.
- ↑ Package Insert, Bio-Rad Liquichek Controls, Bio-Rad ECS Division, Anaheim, CA., 1992.
- ↑ Clinical Diagnosis & Management by Laboratory Methods, 19th edition, J.B. Henry (ed), W.B. Saunders Co., Philadelphia, PA. 1996, pg 11.
- ↑ Lactate Dehydrogenase Total, Body Fluid Laboratory Test Directory ARUP: http://www.aruplab.com/guides/ug/tests/0020505.jsp