protein synthesis elongation

Biochemistry

* see figure 1

• protein synthesis 7
  • substrate: elongation factor 1 + GTP
  • product: eF1-alpha (GTP) + eF1-beta
• protein synthesis 8
  • substrate: eF1-alpha (GTP) + tRNA :amino acyl amino acid residue
  • product: molecular complex - subunits: eF1-alpha (GTP) + tRNA :amino acyl amino acid residue
• protein synthesis 9
  • substrate: molecular complex - subunits: eF1-alpha (GTP) + tRNA :amino acyl amino acid residue + 80S-initiation complex
  • product: 80S-initiation complex :a-site transfer RNA :amino acyl amino acid residue :p-site transfer RNA :amino acyl met + eF1-alpha (GDP) + H2PO4-

* see figure 2

• protein synthesis 10
  • substrate: eF1-alpha (GDP) + eF1-beta
  • product: elongation factor 1 + GDP
• protein synthesis 11
  • enzyme: peptidyl transferase
  • substrate: 80S-initiation complex :a-site transfer RNA :amino acyl amino acid residue :p-site transfer RNA :amino acyl met
  • product: 80S-initiation complex :a-site transfer RNA :amino acyl peptide amino acid residue - met :p-site transfer RNA :amino acyl unbound

* see figure 3

• protein synthesis 12
  • substrate: 80S-initiation complex :a-site transfer RNA :amino acyl peptide amino acid residue - met :p-site transfer RNA :amino acyl unbound + elongation factor 2
  • product: 80S-elongation complex :a-site unbound :p-site transfer RNA :amino acyl peptide + elongation factor 2 (GDP) + H2PO4-
• protein synthesis 13
  • enzyme: GDP-GTP dissociation stimulator
  • substrate: elongation factor 2 (GDP, GTP)
  • product: elongation factor 2 + GDP

* see figure 4 & figure 7

• protein synthesis 14
  • substrate: molecular complex - subunits: eF1-alpha (GTP) + tRNA :amino acyl amino acid residue + 80S-elongation complex :a-site unbound :p-site transfer RNA :amino acyl peptide
  • product: 80S-elongation complex :a-site transfer RNA :amino acyl amino acid residue :p-site transfer RNA :amino acyl peptide + eF1-alpha (GDP) + H2PO4-

* see figure 5

• protein synthesis 15
  • enzyme: peptidyl transferase
  • substrate: 80S-elongation complex :a-site transfer RNA :amino acyl amino acid residue :p-site transfer RNA :amino acyl peptide
  • product: 80S-elongation complex :a-site transfer RNA :amino acyl peptide :p-site transfer RNA :amino acyl unbound

* see figure 6

• protein synthesis 16
  • substrate: 80S-elongation complex :a-site transfer RNA :amino acyl peptide :p-site transfer RNA :amino acyl unbound + elongation factor 2
  • product: 80S-elongation complex :a-site unbound :p-site transfer RNA :amino acyl peptide + elongation factor 2 (GDP) + H2PO4-

* figures 4 & 7 are the same representing repetition of polymerization

More general terms

• protein synthesis