Retinol dehydrogenase 16 (sterol/retinol dehydrogenase, microsomal NAD+-dependent retinol dehydrogenase 4, RoDH-4, RDH16, RODH4)

Function

• oxidoreductase with a preference for NAD
• oxidizes all-trans-retinol & 13-cis-retinol to the corresponding aldehydes
• higher activity towards CRBP-bound retinol than with free retinol
• oxidizes 3-alpha-hydroxysteroids
• oxidizes androstanediol & androsterone to dihydrotestosterone & androstanedione
• can also catalyze the reverse reaction
• inhibited by citral, perillyl alcohol, geraniol, farnesol & geranyl geraniol

Kinetic parameters:

• KM=0.14 uM for androsterone
• KM=0.22 uM for androstanediol
• KM=0.80 uM for dihydrotestosterone
• KM=0.13 uM for NAD
• KM=190 uM for NADP

Structure

• belongs to the short-chain dehydrogenases/reductases (SDR) family
• not N-glycosylated

Compartment

• microsome membrane

Expression

• highly expressed in adult liver (at protein level)
• detected in endometrium, liver & foreskin
• detected in the spineous layers of adult skin, & at lower levels in basal & granular skin layers
• detected in fetal liver & lung
• transiently up-regulated by retinoic acid

More general terms

• membrane protein
• retinal dehydrogenase

Database

• UniProt: http://www.uniprot.org/uniprot/O75452.html