Prolyl 3-hydroxylase 3 (leprecan-like protein 2, protein B, LEPREL2, P3H3)

Function

• prolyl 3-hydroxylase activity
• catalyzes posttranslational formation of 3-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens, especially collagen type 4 & collagen type 5

Procollagen L-proline + 2-oxoglutarate + O2
                    <-->
procollagen trans-3-hydroxy-L-proline + succinate + CO2

Cofactor: Fe+2/Fe+3, ascorbate

Structure

• belongs to the leprecan family
• contains 1 PKHD domain (prolyl/lysyl hydroxylase domain)
• contains 4 TPR repeats

Compartment

endoplasmic reticulum

Alternative splicing

named isoforms=2

Expression

• weak expression in heart, lung, ovary, skeletal muscle

More general terms

• hydroxylase; monooxygenase
• glycoprotein
• metalloprotein

Database

• UniProt: http://www.uniprot.org/uniprot/Q8IVL6.html