DNA excision repair protein ERCC-6-like ( ATP-dependent helicase ERCC6-like, PLK1-interacting checkpoint helicase, tumor antigen BJ-HCC-15) (ERCC6L PICH)
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Function
- DNA helicase
- acts as an essential component of the spindle assembly checkpoint
- contributes to the mitotic checkpoint by recruiting MAD2 to kinetochores & monitoring tension on centromeric chromatin
- acts as a tension sensor that associates with catenated DNA which is stretched under tension until it is resolved during anaphase
- interacts with PLK1, which phosphorylates it; both proteins are mutually dependent on each other for correct subcellular localization; phosphorylation by PLK1 prevents the association with chromosome arms & restricts its localization to the kinetochore-centromere region
Structure
- belongs to the SNF2/RAD54 helicase family
- contains 1 helicase ATP-binding domain
- contains 1 helicase C-terminal domain
- contains 2 TPR repeats
Compartment
- centromere
- localizes to kinetochores, inner centromeres & thin threads connecting separating chromosomes even during anaphase
- in prometaphase cells, it mostly concentrates in between kinetochores
- in metaphase, it localizes to numerous thin threads that stretch between sister kinetochores of the aligned chromosomes
- evolution from inner centromeres to thin threads takes place in response to tension
- resolution of thin threads requires topoisomerase 2-alpha (TOP2A) after anaphase onset