NSFL1 cofactor p47 (p97 cofactor p47, NSFL1C)

Function

• decreased ATPase activity of VCP
• necessary for fragmentation of Golgi stacks during mitosis & for VCP-mediated reassembly of Golgi stacks after mitosis
• role in VCP-mediated formation of transitional endoplasmic reticulum (putative)
• component of ternary complex containing STX5A, NSFL1C & VCP
• forms homotrimer that binds to one end of VCP homohexamer
  • the complex binds to membranes enriched in phosphatidylethanolamine-containing lipids
  • interaction with VCIP135 leads to dissociation of the complex via ATP hydrolysis by VCP
• binds ubiquitin & mono-ubiquitinated proteins via its N-terminal UBA-like domain when bound to VCP
• phosphorylated during mitosis
  • phosphorylation inhibits interaction with Golgi membranes & is required for fragmentation of the Golgi stacks during mitosis

Structure

contains 1 UBX domain

Compartment

• nucleus, Golgi
• predominantly nuclear in interphase
• bound to the axial elements of sex chromosomes in pachytene spermatocytes
• small proportion is associated with Golgi stacks

Alternative splicing

named isoforms=3

More general terms

• nuclear protein
• phosphoprotein

Database

• OMIM: https://mirror.omim.org/entry/606610
• UniProt: http://www.uniprot.org/uniprot/Q9UNZ2.html