immature colon carcinoma transcript 1 protein; peptidyl-tRNA hydrolase ICT1, mitochondrial; digestion substraction 1; DS-1 (ICT1, DS1)

Function

• peptidyl-tRNA hydrolase component of the mitochondrial large ribosomal subunit
• codon-independent translation release factor that has lost all stop codon specificity & directs the termination of translation in mitochondrion, possibly in case of abortive elongation
• may be involved in the hydrolysis of peptidyl-tRNAs that have been prematurely terminated & thus in the recycling of stalled mitochondrial ribosomes

N-substituted aminoacyl-tRNA + H2O = N-substituted amino acid + tRNA component of the mitochondrial 39S ribosomal subunit

Structure

• belongs to the prokaryotic/mitochondrial release factor family, ICT1 subfamily
• in contrast to other members of the family, lacks the regions that come into close contact with the mRNA in the ribosomal A-site & determine the STOP codon specificity, explaining the loss of codon specificity for translation release factor activity

Compartment

mitochondria

Expression

• down-regulated during the in vitro differentiation of HT29-D4 colon carcinoma cells

More general terms

• hydrolase
• phosphoprotein
• mitochondrial protein

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=3396
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:3396
• OMIM: https://mirror.omim.org/entry/603000
• UniProt: http://www.uniprot.org/uniprot/Q14197.html