fluorescent dye Cy5

Introduction

Fluorescent dye used to label dCTP &/or dUMP in synthesis of cDNA by reverse transcriptase. The reverse transcriptase tends to stutter or slow when it encounters the dye.

Notes

Cy5 is excited by red light:

• diode laser 635 nm
• HeNe laser 633 nm

Peak excitation: 649 nm

Peak emission: 670 nm

Cy5-dCTP (Amersham #PA55021)

More general terms

• fluorescent dye