phencyclidine in urine
Indications
- evaluation of substance abuse &/or poisoning
Reference interval
- Not applicable.
Principle
The Urine PCP pack is used in the DuPont ACA discrete clinical analyzer to qualitatively screen phencyclidine in human urine. It is an adaptation of Syva's homogeneous enzyme immunoassay technique, EMIT. The reagents used in this methodology are matched lots of polyclonal antibody reactive to PCP & the PCP- glucose-6-phosphate dehydrogenase conjugate. The concentration of phencyclidine in the sample determines the amount of PCP - glucose- 6-phosphate dehydrogenase (PCP-G6PD) conjugate that is bound to the antibody reactive to PCP. The unbound conjugate catalyzes the oxidation of glucose-6-phosphate, with the simultaneous reduction of NAD to NADH, more rapidly than does the bound conjugate. The rate of increasing absorbance at 340 nm due to the increase in NADH is related to the concentration of phencyclidine in the sample by means of a calibration curve or a mathematical function.
Clinical significance
The urine phencyclidine assay is used to qualitatively detect phencyclidine in human urine. This test provides only a preliminary analytical test result. A more specific alternate chemical method must be used in order to obtain a confirmed analytical result. Gas Chromatography/Mass Spectrometry (GC/MS) is the preferred confirmatory method. Clinical consideration & professional judgement should be applied to any drug of abuse test result, particularly when preliminary positive results are used.
Specimen
Patient preparation: No special patient preparation is required.
Urine samples should be collected in clean, unbreakable, & leak proof containers. Freshly voided urine specimens should be used. If not analyzed immediately, samples should be stored refrigerated. Refrigerated storage exceeding 3 days, however, may result in positive samples with drug concentrations around 25 ng/mL assaying as falsely negative. Drug-free samples have not been observed to produce positive results following storage.
Samples should be within the pH range of 5-8. Samples with pH outside this range should be adjusted to this range by the addition of 1 N HCL or 1N NaOH before assaying. Specimens may be encountered that display high turbidity. It is recommended that these be centrifuged before analysis.
More general terms
Additional terms
Component of
References
- ↑ ACA IV Discrete Clinical Analyzer Instrument Manual, Volume 1: Operation, DuPont Company, Wilmington, Delaware, 1984.
- ↑ ACA IV Discrete Clinical Analyzer Instrument Manual, Volume 3B: Chemistry, DuPont Company, Wilmington, Delaware, 1984.
- ↑ Drug Confirmation, Phencyclidine, Urine Laboratory Test Directory ARUP: http://www.aruplab.com/guides/ug/tests/0090366.jsp
- ↑ Panel of 10 tests Laboratory Test Directory ARUP: http://www.aruplab.com/guides/ug/tests/0090448.jsp
- ↑ Panel of 11 tests Laboratory Test Directory ARUP: http://www.aruplab.com/guides/ug/tests/0090449.jsp
- ↑ Panel of 35 tests Laboratory Test Directory ARUP: http://www.aruplab.com/guides/ug/tests/0090453.jsp
- ↑ Panel of 13 tests Laboratory Test Directory ARUP: http://www.aruplab.com/guides/ug/tests/0090454.jsp
- ↑ Panel of 8 tests Laboratory Test Directory ARUP: http://www.aruplab.com/guides/ug/tests/0090491.jsp
- ↑ Panel of 9 tests Laboratory Test Directory ARUP: http://www.aruplab.com/guides/ug/tests/0090492.jsp
- ↑ Panel of 6 tests Laboratory Test Directory ARUP: http://www.aruplab.com/guides/ug/tests/0092182.jsp
- ↑ Panel of 6 tests Laboratory Test Directory ARUP: http://www.aruplab.com/guides/ug/tests/0092183.jsp
- ↑ Panel of 8 tests Laboratory Test Directory ARUP: http://www.aruplab.com/guides/ug/tests/0092184.jsp
- ↑ Panel of 8 tests Laboratory Test Directory ARUP: http://www.aruplab.com/guides/ug/tests/0092185.jsp
- ↑ Panel of 10 tests Laboratory Test Directory ARUP: http://www.aruplab.com/guides/ug/tests/0092186.jsp
- ↑ Panel of 10 tests Laboratory Test Directory ARUP: http://www.aruplab.com/guides/ug/tests/0092187.jsp