Enolase-phosphatase E1 (EC 3.1.3.77, 2,3-diketo-5-methylthio-1- phosphopentane phosphatase, MASA homolog) (ENOPH1 MASA MSTP145)

Function

• bifunctional enzyme that enolizes substrate to form 2-hydroxy-5-(methylthio)-3-oxopent-1-enyl phosphate, which is dephosphorylated to form the acireductone 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one
• methionine salvage pathway

5-(methylthio)-2,3-dioxopentyl phosphate + H2O
                  <-->
1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + phosphate

Cofactor: binds 1 Mg+2 per subunit

Structure

• belongs to the HAD-like hydrolase superfamily, MasA family

Alternative splicing

named isoforms=2

More general terms

• phosphomonoesterase
• enolase (2-phospho-D-glycerate hydrolase)

Database

• UniProt: http://www.uniprot.org/uniprot/Q9UHY7.html