bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 2; glucosaminyl N-deacetylase/N-sulfotransferase 2; NDST-2; N-heparan sulfate sulfotransferase 2; N-HSST 2 [includes: heparan sulfate N-deacetylase 2; heparan sulfate N-sulfotransferase 2] (NDST2, HSST2)

Function

• essential bifunctional enzyme that catalyzes both N-deacetylation & the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate
• modifies GlcNAc-GlcA dissacharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis
• role in determining extent & pattern of sulfation of heparan sulfate
• glycan metabolism; heparan sulfate biosynthesis
• glycan metabolism; heparin biosynthesis

3'-phosphoadenylyl sulfate + [heparansulfate]-glucosamine
                 <-->
adenosine 3',5'-bisphosphate +  [heparansulfate]-N-sulfoglucosamine

Kinetic parameters:

• KM=4.7 uM for K5 polysaccharide
• KM=0.76 uM for N-acetylated HS-II

Structure

• monomer (putative)
• belongs to the sulfotransferase 1 family, NDST subfamily

Compartment

Golgi membrane

Alternative splicing

named isoforms=2

Notes

• the presence of 4 different heparan sulfate N-deacetylase/N-sulfotransferase enzymes in mammals, as well as differences in their enzyme activity suggest that some initiate heparan sulfate modification/ sulfation reactions, whereas others later on fill in or extend already modified heparan sulfate sequences

More general terms

• sulfotransferase
• glycoprotein
• membrane protein

Database

• UniProt: http://www.uniprot.org/uniprot/P52849.html
• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=8509
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:8509
• OMIM: https://mirror.omim.org/entry/603268