bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1; glucosaminyl N-deacetylase/N-sulfotransferase 1; NDST-1; [heparan sulfate]-glucosamine N-sulfotransferase 1; HSNST 1; N-heparan sulfate sulfotransferase 1; N-HSST 1 [includes: heparan sulfate N-deacetylase 1; heparan sulfate N-sulfotransferase 1] (NDST1, HSST, HSST1)

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Function

essential bifunctional enzyme
catalyzes both N-deacetylation & N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate
modifies the GlcNAc-GlcA dissacharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis
plays a role in determining the extent & pattern of sulfation of heparan sulfate
role in biosynthesis of heparan sulfate that can ultimately serve as L- selectin ligands, thereby playing a role in inflammatory response
glycan metabolism; heparan sulfate biosynthesis
glycan metabolism; heparin biosynthesis

3'-phosphoadenylyl sulfate + [heparansulfate]-glucosamine
               <-->
adenosine 3',5'-bisphosphate +  [heparan sulfate]-N-sulfoglucosamine

Kinetic parameters:

Golgi membrane s

the presence of 4 different heparan sulfate N-deacetylase/N-sulfotransferase enzymes in mammals, as well as differences in their enzyme activity suggest that some initiate heparan sulfate modification/ sulfation reactions, whereas others later on fill in or extend already modified heparan sulfate sequences