Multidrug & toxin extrusion protein 2 (MATE-2, hMATE-2, solute carrier family 47 member 2, kidney-specific H+/organic cation antiporter, SLC47A2, MATE2)

Function

• solute transporter for tetraethylammonium (TEA), 1-methyl-4-phenylpyridinium (MPP), cimetidine, N-methylnicotinamide, metformin, creatinine, guanidine, procainamide, topotecan, estrone sulfate, acyclovir, & ganciclovir
• responsible for the secretion of cationic drugs across brush border membranes

Kinetic parameters:

• KM=0.76 mM for TEA
• KM=0.11 mM for MPP
• KM=0.12 mM for cimetidine
• KM=1.98 mM for metformin
• KM=4.2 mM for guanidine
• KM=1.58 mM for procainamide
• KM=0.06 mM for topotecan
• KM=0.85 mM for estrone sulfate
• KM=4.32 mM for acyclovir
• KM=4.28 mM for ganciclovir
• Vmax=0.88 nmol/min/mg enzyme toward TEA
• Vmax=0.575 nmol/min/mg enzyme toward MPP
• Vmax=0.115 nmol/min/mg enzyme toward cimetidine
• Vmax=0.845 nmol/min/mg enzyme toward metformin
• Vmax=0.58 nmol/min/mg enzyme toward guanidine
• Vmax=3.385 nmol/min/mg enzyme toward procainamide
• Vmax=0.13 nmol/min/mg enzyme toward topotecan
• Vmax=0.425 nmol/min/mg enzyme toward estrone sulfate
• Vmax=0.945 nmol/min/mg enzyme toward acyclovir
• Vmax=0.805 nmol/min/mg enzyme toward ganciclovir
• Optimum pH is 9.0. active from pH 6 to 9

Structure

belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Compartment

• membrane
• localized at the brush border membranes of proximal tubules

Alternative splicing

named isoforms=6

Expression

• isoform 3 is predominantly expressed in kidney
• isoform 6 is expressed in brain

More general terms

• transmembrane 13 protein
• solute carrier family 47 (SLC47, SLC47A)

Database

• UniProt: http://www.uniprot.org/uniprot/Q86VL8.html