ubiquitin carboxyl-terminal hydrolase 3; deubiquitinating enzyme 3; ubiquitin thiolesterase 3; ubiquitin-specific-processing protease 3 (USP3)

Function

• deubiquitinates monoubiquitinated target proteins including histone H2A & H2B
• required for proper progression through S phase & subsequent mitotic entry
• may regulate the DNA damage response (DDR) checkpoint through deubiquitination of H2A at DNA damage sites
• associates with the chromatin
• thiol-dependent hydrolysis of ester, thioester, amide, peptide & isopeptide bonds formed by the C-terminal Gly of ubiquitin
• interacts (via UBP-type domain) with H2A; the interaction is less efficient than with monoubiquitinated H2A

Structure

• both protease activity & an intact Zn+2 finger are required for H2A monodeubiquitination
• belongs to the peptidase C19 family, USP3 subfamily
• contains 1 UBP-type Zn+2 finger

Compartment

• nucleus
• localizes preferentially with monoubiquitinated H2A to chromatin

Expression

• expressed in all tissues examined
• strongest expression in pancreas

More general terms

• ubiquitin C-terminal hydrolase
• nuclear protein
• zinc finger protein
• phosphoprotein

Additional terms

• ubiquitin (UBCEP2, UBB, UBC)

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=9960
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:9960
• OMIM: https://mirror.omim.org/entry/604728
• UniProt: http://www.uniprot.org/uniprot/Q9Y6I4.html