NAD kinase; poly(P)/ATP NAD kinase (NADK)

Function

• phosphorylates NAD+

ATP + NAD+ = ADP + NADP+

Cofactor: divalent metal cations

Kinetic parameters:

• KM=3.3 mM for ATP
• KM=0.54 mM for NAD
• Vmax=6.7 umol/min/mg enzyme
• pH dependence: optimum pH is 7.5
• temperature dependence: optimum temperature is 55 degrees C

Structure

• belongs to the NAD kinase family

Expression

• widely expressed
• not detected in skeletal muscle

More general terms

• kinase or ATP-phosphotransferase

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=65220
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:65220
• OMIM: https://mirror.omim.org/entry/611616
• UniProt: http://www.uniprot.org/uniprot/O95544.html