aldo-keto reductase family 1, member C2; dihydrodiol dehydrogenase-2; bile acid binding protein; 3-alpha hydroxysteroid dehydrogenase type 3; trans-1,2-dihydrobenzene-1,2-diol dehydrogenase; 3-alpha-HSD3; chlordecone reductase homolog HAKRD; dihydrodiol dehydrogenase/bile acid-binding protein; DD/BABP (AKR1C2, DD2, DDH2)

Function

• conversion steroid hormones into the 3alpha/5alpha & 3alpha/5beta-tetrahydrosteroids
• works in concert with the 5alpha/5beta-steroid reductases
• catalyzes inactivation 5-alpha-dihydrotestosterone to 5-alpha-androstane-3alpha,17beta-diol
• high bile-binding ability

Inhibition:

• hexestrol with an IC(50) of 2.8 uM
• 1,10-phenanthroline with an IC(50) of 2100 uM
• 1,7- phenanthroline with an IC(50) of 1500 uM
• flufenamic acid with an IC(50) of 0.9 uM
• indomethacin with an IC(50) of 75 uM
• ibuprofen with an IC(50) of 6.9 uM
• lithocholic acid with an IC(50) of 0.07 uM
• ursodeoxycholic acid with an IC(50) of 0.08 uM
• chenodeoxycholic acid with an IC(50) of 0.13 uM

Kinetic parameters:

• KM=260 uM for (s)-tetralol
• KM=520 uM for (s)-indan-1-ol
• KM=5000 uM for benzene dihydrodiol
• KM=1 uM for 5-beta-pregnane-3-alpha,20-alpha-diol
• KM=208 uM for 9-alpha,11-beta-PGF2
• KM=0.3 uM for 5-beta-androstane-3,17-dione
• KM=79 uM for PGD2

trans-1,2-dihydrobenzene-1,2-diol + NADP+
     <-->
catechol + NADPH

androsterone + NAD+/NADP+
        <-->
5-alpha-androstane-3,17-dione + NADH/NADPH

Structure

belongs to the aldo/keto reductase family

Compartment

cytoplasm (putative)

More general terms

• trans-1,2-dihydrobenzene-1,2-diol dehydrogenase
• aldo-keto reductase

Database

• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:1646
• OMIM: https://mirror.omim.org/entry/600450
• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=1646
• UniProt: http://www.uniprot.org/uniprot/P52895.html