polypeptide N-acetylgalactosaminyltransferase 16; polypeptide galNAc transferase-like protein 1; galNAc-T-like protein 1; pp-GaNTase-like protein 1; polypeptide N-acetylgalactosaminyltransferase-like protein 1; protein-UDP acetylgalactosaminyltransferase-like protein 1; UDP-galNAc:polypeptide N-acetylgalactosaminyltransferase-like protein 1 (GALNT16 GALNTL1 KIAA1130)

Function

• glycosylation
• catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, transfer of an N-acetyl-D-galactosamine residue to a Ser or Thr on the protein

UDP-N-acetyl-D-galactosamine + polypeptide
                   <-->
UDP + N-acetyl-D-galactosaminyl-polypeptide

Cofactors: Mn+2, Ca+2

Structure

• 2 conserved domains in the glycosyltransferase region
  • N-terminal domain (domain A, also called GT1 motif), involved in Mn+2 coordination & substrate binding
  • C-terminal domain (domain B, also called Gal/GalNAc-T motif) involved in catalysis & UDP-Gal binding
• ricin B-type lectin domain binds to GalNAc & contributes to glycopeptide specificity
• belongs to the glycosyltransferase 2 family, GalNAc-T subfamily.
• contains 1 ricin B-type lectin domain

Compartment

Golgi membrane

Alternative splicing

named isoforms=2

More general terms

• polypeptide N-acetylgalactosaminyltransferase

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=57452
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:57452
• UniProt: http://www.uniprot.org/uniprot/Q8N428.html