potassium (K+) in body fluid

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Principle

The Kodak Ektachem Clinical Chemistry Slide (K+) quantitatively measures potassium in serum, plasma or urine.

The Kodak Ektachem Clinical Chemistry Slide (K+) is a dry, multilayered, analytical element that uses direct potentiometry for measurement of ionic potassium. The slide consists of two ion-selective electrodes, each containing valinomycin (an ionophore for potassium), a reference layer, & a silver & silver chloride layer coated on a plastic support.

Application of 10 uL of patient sample & 10 uL of Kodak Ektachem Electrolyte Reference Fluid to separate halves of the slide results in migration of both fluids towards the center of the paper bridge. A stable liquid junction is formed connecting the reference electrode to the sample indicator electrode.

Each electrode produces an electrical potential in response to the activity of potassium applied to it. The potential difference poised between the two half electrodes is proportional to the potassium concentration in the sample.

Clinical significance

Specimen

No special patient preparation is required.

See K+, K+ in serum, K+ in urine

Minimum sample size 0.5 milliliter: with an optimum size of 1.0 milliliters or larger.

More general terms

More specific terms

Additional terms

References

  1. Tietz, N. W.: Electrolytes, in Textbook of Clinical Chemistry. Philadelphia, W. B. Saunders Co., p. 617, 1987.
  2. Friedman R. B., Young D. S.: Effects of Disease on Clinical Laboratory Tests, Washington, D. C.; AACC Press, 1990.
  3. Package Inserts: Kodak Ektachem Clinical Chemistry Slide (K+) Kodak Clinical Products, Rochester, New York, 1992.
  4. Kodak Ektachem 700 Analyzer Operator's Manual, Kodak Clinical Products, Rochester, New York
  5. Kodak Ektachem Training Manual, Kodak Clinical Products, Rochester, New York, 1993.
  6. Mayo Internal Medicine Board Review, 1998-99, Prakash UBS (ed) Lippincott-Raven, Philadelphia, 1998, pg 601.
  7. Clinical Diagnosis & Management by Laboratory Methods, 19th edition, J.B. Henry (ed), W.B. Saunders Co., Philadelphia, PA. 1996, pg 11.
  8. Potassium, Body Fluid Laboratory Test Directory ARUP: http://www.aruplab.com/guides/ug/tests/0020155.jsp

Patient information

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