glycoprotein endo-alpha-1,2-mannosidase (mandaselin, hEndo, MANEA)

Function

• undergoes proteolytic cleavage in the C-terminal region
• hydrolysis of the terminal alpha-D-glucosyl-(1,3)-D-mannosyl unit from the GlcMan(9)(GlcNAc)(2) oligosaccharide component of the glycoprotein produced in the Golgi membrane

Kinetic parameters:

• Optimum pH is 6.2
• Optimum temperature 37 degrees C

Structure

belongs to the glycosyl hydrolase 99 family

Compartment

Golgi membrane

Expression

• highly expressed in the liver & kidney
• expressed at lower levels in muscle, pancreas, heart, placenta, lung & brain

More general terms

• alpha-mannosidase (MANA)
• membrane protein

Database

• UniProt: http://www.uniprot.org/uniprot/Q5SRI9.html