flavoprotein monooxygenase 2; dimethylaniline oxidase 2; dimethylaniline monooxygenase (N-oxide forming) 2; pulmonary flavin-containing monooxygenase 2 (FMO2, FMO1B1)

Function

• catalyzes the N-oxidation of certain primary alkylamines to their oximes via an N-hydroxylamine intermediate
• inactive toward certain tertiary amines, such as imipramine or chloropromazine
• can catalyze the S-oxidation of methimazole
• truncated form is catalytically inactive
• truncated form is probably unable to fold correctly & is rapidly degraded

N,N-dimethylaniline + NADPH + O2
     <-->
N,N- dimethylaniline N-oxide + NADP+ + H2O

Cofactor: FAD, Mg+2

Structure

belongs to the FMO family

Compartment

• microsome membrane
• endoplasmic reticulum membrane

Expression

• expressed predominantly in lung
• expressed to much lesser extent in kidney

Polymorphism

• two alleles; one major, FMO2X472, (truncated form) & one minor, FMO2Q472 (full-length form similar to the protein found in other mammals)
• a nonsense mutation transforms the Gln-472 in a premature stop codon
• the truncated protein is catalytically inactive
• the minor allele is present at a frequency of approximately 4% in the sample of population of African descent

More general terms

• flavoprotein mixed function oxidase
• membrane protein

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=2327
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:2327
• OMIM: https://mirror.omim.org/entry/603955
• UniProt: http://www.uniprot.org/uniprot/Q99518.html