Ca+2-independent phospholipase A2-gamma (intracellular membrane-associated Ca+2-independent phospholipase A2 gamma, iPLA2-gamma, patatin-like phospholipase domain-containing protein 8, PNPLA8, IPLA22, IPLA2G, BM-043)

Function

• Ca+2-independent phospholipase A2
• catalyzes hydrolysis of sn-2 position of glycerophospholipids, PtdSer & to a lower extent PtdCho
• cleaves membrane phospholipids
• inhibited by E-6-bromomethylene-3-1-naphthalenyl-2H- tetrahydropyran-2-one (BEL)

2-lysophosphatidylcholine + H2O <--> glycerophosphocholine + carboxylate

Kinetic parameters:

• Optimum pH is 8.0

Structure

contains 1 patatin domain

Compartment

• membrane (putative)
• perinuclear, endoplasmic reticulum & Golgi

Alternative splicing

named isoforms=2

Expression

• expressed in parenchymal tissues including heart, skeletal muscle, placenta, brain, liver & pancreas
• also expressed in bronchial epithelial cells & kidney
• highest expression is observed in skeletal muscle & heart

More general terms

• glycoprotein
• phospholipase A2; phosphatidylcholine 2-acylhydrolase (PLA2)

Database

• UniProt: http://www.uniprot.org/uniprot/Q9NP80.html