bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 3; glucosaminyl N-deacetylase/N-sulfotransferase 3; NDST-3; hNDST-3; N-heparan sulfate sulfotransferase 3; N-HSST 3; includes: heparan sulfate N-deacetylase 3; heparan sulfate N-sulfotransferase 3 (NDST3, HSST3, UNQ2544/PRO4998)

Function

• bifunctional enzyme in heparin/heparan sulfate synthesis
• catalyzes both N-deacetylation & N-sulfation of glucosamine of glycosaminoglycan in heparan sulfate.
• modifies GlcNAc-GlcA dissacharide repeating sugar backbone to make N-sulfated heparosan, substrate for later modifications in heparin biosynthesis
• high deacetylase activity but low sulfotransferase activity

3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine
              <-->
adenosine 3',5'-bisphosphate +  [heparan sulfate]-N-sulfoglucosamin

Structure

• monomer
• belongs to the sulfotransferase 1 family, NDST subfamily

Compartment

Golgi

Alternative splicing

named isoforms=2

Expression

• expressed in brain, kidney, liver, fetal & adult lung, adult pancreas, placenta, fetal spleen, fetal thymus
• Not detected in adult/fetal heart or skeletal muscle

Comparative biology

• presence of 4 different heparan sulfate N-deacetylase/ N-sulfotransferase enzymes in mammals, & differences in enzyme activity suggest that some initiate heparan sulfate modification/sulfation reactions, whereas others extend already modified heparan sulfate sequences

More general terms

• sulfotransferase
• hydrolase
• glycoprotein

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=9348
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:9348
• OMIM: https://mirror.omim.org/entry/603950
• UniProt: http://www.uniprot.org/uniprot/O95803.html