acyl-coenzyme A synthetase ACSM1, mitochondrial (acyl-CoA synthetase medium-chain family member 1, middle-chain acyl-CoA synthetase 1, butyrate-CoA ligase 1, butyryl coenzyme A synthetase 1, lipoate-activating enzyme, ACSM1, BUCS1, LAE, MACS1)

Function

• medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro)
• acts on acids from C(4) to C(11) & on the corresponding 3-hydroxy- & 2,3- or 3,4- unsaturated acids (in vitro)
• functions as GTP-dependent lipoate-activating enzyme that generates the substrate for lipoyltransferase (putative)

ATP + an acid + CoA
             <-->
AMP + diphosphate + acyl-CoA

GTP + lipoate <--> diphosphate + lipoyl-GMP

Cofactor: Mg+2 (putative)

Structure

• belongs to the ATP-dependent AMP-binding enzyme family

Compartment

mitochondrial matrix (putative)

Alternative splicing

named isoforms=2

More general terms

• acyl coenzyme-A [CoA] synthetase or acyl coenzyme-A [CoA] ligase
• mitochondrial protein

Database

• UniProt: http://www.uniprot.org/uniprot/Q08AH1.html