ATP-dependent Clp protease proteolytic subunit, mitochondrial; endopeptidase Clp (CLPP)

Function

• Clp cleaves peptides in various proteins in a process that requires ATP hydrolysis
• Clp may be responsible for a fairly general & central housekeeping function rather than for the degradation of specific substrates
• hydrolysis of proteins to small peptides in the presence of ATP & Mg+2
• alpha-casein is the usual test substrate
• in the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec & Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- & -Tyr-|-Trp bonds also occurs)
• tetradecamer that assembles into a two heptameric rings with a central cavity

Structure

• belongs to the peptidase S14 family

Compartment

mitochondrial matrix

Expression

• predominantly expressed in skeletal muscle
• intermediate levels in heart, liver & pancreas. low in brain, placenta, lung & kidney

More general terms

• protein subunit
• mitochondrial protein
• phosphoprotein

Component of

• endopeptidase Clp (ATP-dependent Clp protease)

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=8192
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:8192
• OMIM: https://mirror.omim.org/entry/601119
• UniProt: http://www.uniprot.org/uniprot/Q16740.html