serine/threonine protein kinase Nek7; never in mitosis A-related kinase 7; NimA-related protein kinase 7 (NEK7)
Jump to navigation
Jump to search
Function
- serine/threonine protein kinase
- role in mitotic cell cycle progression
- required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation & cytokinesis
- phosphorylates RPS6KB1
- binding to NEK9 stimulates its activity by releasing the autoinhibitory function of Tyr-97
- phosphorylation at Ser-195 required for its activation
- interacts with NEK9
Structure
- monomer
- displays an autoinhibited conformation:
- Tyr-97 side chain points into the active site, interacts with the activation loop, & blocks the alphaC helix
- the autoinhibitory conformation is released upon binding with NEK9
- the NTE (N-terminal extension) motif is a structural component of the catalytic domain & thus contributes to activity
- belongs to the protein kinase superfamily, NEK Ser/Thr protein kinase family, NIMA subfamily
- contains 1 protein kinase domain
Compartment
- nucleus, cytoplasm
- cytoskeleton, spindle pole, centrosome
- present at centrosome throughout the cell cycle
- also detected at spindle midzone of the anaphase cells & eventually concentrates at the midbody
Alternative splicing
named isoforms=2
Expression
- highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte & spleen
- lower expression in ovary, prostate & kidney
- not expressed in small intestine