ubiquitin-activating enzyme E1 homolog (ubiquitin-like modifier-activating enzyme 7, UBA7, UBE2, UBE1L)

Function

• activates ubiquitin by first adenylating with ATP its carboxy-terminal Gly & thereafter linking this Gly to the side chain of Cys in E1, yielding an ubiquitin-E1 thioester & free AMP
• protein degradation; protein ubiquitinylation
• binds & is involved in the conjugation of G1P2/ISG15

Structure

• monomer (putative)
• belongs to the ubiquitin-activating E1 family
• there are 2 active sites within the E1 molecule, allowing it to accommodate 2 ubiquitin moieties at a time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site

Expression

• expressed in a variety of normal & tumor cell types

Pathology

• expression is diminished in lung cancer cell lines

More general terms

• acyltransferase

Database

• UniProt: http://www.uniprot.org/uniprot/P41226.html
• OMIM: https://mirror.omim.org/entry/191325
• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=7318