pyridoxal phosphate phosphatase PHOSPHO2 (PHOSPHO2)

Function

• phosphatase with high activity toward pyridoxal phosphate
• much lower activity toward pyrophosphate, phosphoethanolamine, phosphocholine, phospho-l-tyrosine, fructose-6-phosphate, p-nitrophenyl phosphate, & h-glycerophosphate

pyridoxal 5'-phosphate + H2O  <-->  pyridoxal + phosphate

Cofactor: Mg+2 (putative)

Kinetic parameters:

• KM=45.5 uM for pyridoxal 5'-phosphate
• Vmax=633 nmol/min/mg enzyme with pyridoxal 5'-phosphate as substrate

Structure

• belongs to the HAD-like hydrolase superfamily, PHOSPHO family

More general terms

• pyridoxal phosphate phosphatase

Database

• UniProt: http://www.uniprot.org/uniprot/Q8TCD6.html