beta-1,4-galactosyltransferase 2; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 2; UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 2 (B4GALT2)

Function

• role in glycosylation
• synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids
• can produce lactose
• protein modification; protein glycosylation

UDP-galactose + D-glucose => UDP + lactose
UDP-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
  => UDP + beta-D-galactosyl-1,4-N-acetyl-beta-D- glucosaminylglycopeptide
UDP-galactose + N-acetyl-D-glucosamine
  => UDP + N-acetyllactosamine

Cofactor: Mn+2 (putative)

Kinetic parameters:

• KM=71 uM for GlcNAc-B-S-pNP

Structure

belongs to the glycosyltransferase 7 family

Compartment

• Golgi stack membrane
• trans cisternae of Golgi stack

Alternative splicing

named isoforms=2

Expression

• weakly expressed in various tissues
• highest expression in prostate, testis, ovary, intestine, muscle, & in fetal brain

More general terms

• beta-1,4-galactosyltransferase

Database

• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:8704
• OMIM: https://mirror.omim.org/entry/604013
• UniProt: http://www.uniprot.org/uniprot/O60909.html