cytosolic endo-beta-N-acetylglucosaminidase (ENgase)

Function

• endoglycosidase
• releases N-glycans from glycoproteins by cleaving the beta-1,4-glycosidic bond in the N,N'-diacetylchitobiose core
• involved in the processing of free oligosaccharides in the cytosol
• endohydrolysis of the N,N'-diacetylchitobiosyl unit in high-mannose glycopeptides & glycoproteins containing the -(man(GlcNAc)(2))Asn-structure
• one N-acetyl-D-glucosamine residue remains attached to the protein; the rest of the oligosaccharide is released intact

Structure

• belongs to the glycosyl hydrolase 85 family
• contains 1 BRCT domain

Compartment

cytoplasm, cytosol (putative)

Alternative splicing

named isoforms=3

Expression

• widely expressed -expressed at higher level in thymus & spleen

More general terms

• N-acetylglucosaminidase

Database

• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:6477
• UniProt: http://www.uniprot.org/uniprot/Q8NFI3.html