surface plasmon resonance

Introduction

No radioactive label.

Smallest protein for which technique successfully employed 180,000 daltons (2003).

ligand is attached to the sensor chip

gold-dextran surface (maximum 400 runs on same surface)

protein in sample binds to ligand

changes in refractive index measured

• resonance of electrons in gold excited by red light
• ligand binding effect electron delocalization in some manner as to affect refractive index

Gives on & off rates, kD

More general terms

• biochemistry