hydroxylysine kinase; 5-hydroxy-L-lysine kinase; aminoglycoside phosphotransferase domain-containing protein 1 (HYKK, AGPHD1)

Function

• catalyzes GTP-dependent phosphorylation of 5-hydroxy-L-lysine

GTP + 5-hydroxy-L-lysine = GDP + 5-phosphonooxy-L-lysine

Kinetic parameters:

• KM=280 uM for (5R)-5-hydroxy-L-lysine (2 mM ATP)
• KM=7.6 uM for (5R)-5-hydroxy-L-lysine (0.2 mM GTP)
• KM=810 uM for ATP (0.5 mM (5R)-5-hydroxy-L-lysine)
• KM=3.5 uM for GTP (0.5 mM (5R)-5-hydroxy-L-lysine)
• Vmax=4.95 umol/min/mg enzyme toward (5R)-5-hydroxy-L-lysine (2 mM ATP)
• Vmax=0.49 umol/min/mg enzyme toward (5R)-5-hydroxy-L-lysine (0.2 mM GTP)
• Vmax=5.28 umol/min/mg enzyme toward ATP (0.5 mM (5R)-5-hydroxy-L-lysine)
• Vmax=0.57 umol/min/mg enzyme toward GTP (0.5 mM (5R)-5-hydroxy-L-lysine)

Structure

• belongs to the aminoglycoside phosphotransferase family

Compartment

cytoplasm (putative)

Alternative splicing

named isoforms=3

Notes

• variant in position: 165:V->I (in dbSNP:4380026).

More general terms

• kinase or ATP-phosphotransferase

Database

• Entrez gene: http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=123688
• Kegg: http://www.genome.jp/dbget-bin/www_bget?hsa:123688
• OMIM: https://mirror.omim.org/entry/614681
• UniProt: http://www.uniprot.org/uniprot/A2RU49.html