urea in body fluid
Principle
The Kodak Ektachem Clinical Chemistry Slide (BUN/UREA) is a dry, multilayered analytical element coated on a clear polyester support.
A 10 uL drop of patient sample is deposited on the slide & is evenly distributed by the spreading layer. Water & non- proteinaceous components then travel to the underlying reagent layer where the urease reaction generates ammonia. The ammonia then diffuses through the semipermeable membrane to react with an ammonia indicator.
The semipermeable membrane allows only ammonia to pass through to the color-forming layer where it reacts with the indicator.
After a fixed incubation period, the reflection density of the dye is measured spectrophotometrically through the polyester support using the white background of the spreading layer as a diffuse reflector
Urease H2NCONH2 + H2O -----------> 2NH3 + CO2
NH3 + Ammonia Indicator ----------> Dye
Specimen
- specimens are collected in a red top vacutainer & should be separated immediately from the cells after collection
- minimum sample size 0.5 milliliter: with an optimum size of 1.0 mL or larger
More general terms
More specific terms
- urea in amniotic fluid
- urea in dialysis fluid
- urea in gastric fluid
- urea in peritoneal fluid
- urea in pleural fluid
- urea in serum/plasma//blood
- urea in vitreous fluid
Additional terms
References
- ↑ Urea Nitrogen, Body Fluid Laboratory Test Directory ARUP: http://www.aruplab.com/guides/ug/tests/0020183.jsp