nerve fiber teasing

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Indications

Procedure

  • performed on prestained proximodistally oriented portions of peripheral nerves
  • specimens about 10 mm long are stained for 24-48 hours in Sudan black & then transferred to glycerin, where, using a pair of fine forceps & a stereomicroscope, they are separated into smaller fiber bundles from which single fibers are isolated

More general terms

References

  1. Krinke GJ et al Teased-Fiber Technique for Peripheral Myelinated Nerves: Methodology and Interpretation Toxicol Pathol 2000 28: 113 http://tpx.sagepub.com/content/28/1/113.full.pdf